confocal projection of Vasa protein expression in primordial germ cells (PGCs) in Parhyale hawaineis. Scale bar = 5um. (photo Extavour)

A. ovaries from a young female; some oocytes are vitellogenic but all are in early stages. A’. DAPI image of A. B. DAPI image of ovary containing primarily late stage oocytes. C. ovary from an older female containing primarily late stage oocytes. C’. DAPI image of C. (photo Extavour)

3D reconstruction made from confocal sections of the forming dorsal organ of a Parhyale hawaiensis embryo.
(photo Extavour)

3D reconstruction of Parhyale hawaiensis germ cells at the germ band stage of embryogenesis, before the split between germ cells of the left and right gonad. Pink = Vasa protein; blue = nuclei. (photo Extavour)

2D projection of confocal stack of a late Parhyale hawaiensis embryo stained with anti-muscle myosin (red)
(photo Extavour)

merge of the previous two images
(photo Extavour)

2D projection of confocal stack of a late Parhyale hawaiensis embryo stained with conjugated phalloidin (green) (photo Extavour)

Germ band stage embryo of Parhyale hawaiensis showing PGCs in a single cluster. green = nuclei; red = anti-Vasa antibody (germ cells) (photo Extavour)

Late stage embryo of Parhyale hawaiensis showing one of the two lateral PGC clusters. green = nuclei; red = anti-Vasa antibody (germ cells) (photo Extavour)

ectodermal cell of an Parhyale hawaiensis early cleavage stage embryo in metaphase. red = alpha tubulin;
blue = DNA (photo Extavour)

ectodermal cell of a Parhyale hawaiensis early cleavage stage embryo in anaphase. red = alpha tubulin;
blue = DNA (photo Extavour)

Left: in situ hybridization for vasa, showing two clumps of migrating germ cells at a late germ band stage.

Right: DAPI stained image of the same embryo.

(photo Linsler)