Animal Culture Protocols

• Cockroach and Cricket diet
• Gryllus bimaculatus culture
• Parhyale hawaiensis culture

Tissue Analysis Protocols

• Crustacean antibody staining
• In situ hybridisation – Gryllus embryos Mar08
• In situ hybridisation - Gryllus embryos May10
• PMN Fixation Buffer
• Cryo Sectioning
• Xgal stain fly tissue
• Parhyale immunohistochemistry
• Parhyale fixation

Other Protocols

• Parhyale hawaiensis staging table
• Schistocerca gregaria staging table
• FK Gryllus egg injections
• How to image Gryllus germbands on the stereoscope
• tracer dye spectra (tif)

Molecular Biology Protocols

• In situ probe synthesis 1
• In situ probe synthesis 2
• RNA extraction with Trizol
• dsRNA synthesis Feb10
• Ph Western_Blotting
• QIAGEN Midi Preps

Transcriptome Protocols

• Adaptors & primers 454 sequencing
• Gb Adaptors

Bioinformatics Tools

• Python Transcriptome Analysis Tools
• Perl Transcriptome Analysis Tools